?↑HACS↑?

文章标题：

Amammaliantranslationinitiationfactorcansubstituteforitsyeasthomologueinvivo.

核心内容：

翻译起始因子4E(EIF-4E)参与真核细胞mRNA5‘端帽子结构的识别，促进核糖体结合。随后，额外的启动因子介导了mRNA的核糖体扫描和启动子Aug的识别(Shatkin，A.J.()Cell40,-；Rhoads，R.E.()TrendsBiochem)。SCI。13，52-56；Edery，I.，Pelletier，J.和Sonenberg，N.()，《基因表达的翻译调控》(Ilan，J.，编辑)第-页，PlenumPublishingCorp.，纽约)。在这里，我们发现启动因子4E在单细胞真核生物酿酒酵母和哺乳动物之间功能上是保守的。虽然这两种因子的氨基酸同源性仅为33%，但小鼠eIF-4E可以在体内替代酵母eIF-4E，而不会对细胞活力、生长和交配产生重大影响。这一发现为研究真核细胞翻译起始因子eIF-4E的结构-功能关系提供了新的实验策略。原文摘要：

Thetranslationinitiationfactor4E(eIF-4E)isinvolvedintherecognitionofthecapstructureatthe5endofeukaryoticmRNAandfacilitatesribosomebinding.Subsequently,additionalinitiationfactorsmediateribosomalscanningofmRNAandinitiatorAUGrecognition(Shatkin,A.J.()Cell40,-;Rhoads,R.E.()TrendsBiochem.Sci.13,52-56;Edery,I.,Pelletier,J.,andSonenberg,N.()inTranslationalRegulationofGeneExpression(Ilan,J.,ed)pp.-,PlenumPublishingCorp.,NewYork).Weshowherethatinitiationfactor4EisfunctionallyconservedbetweentheunicellulareukaryoteSaccharomycescerevisiaeandmammals.Althoughtheaminoacididentityofthefactorsfrombothspeciesislimitedtoonly33%,mouseeIF-4EcansubstituteforyeasteIF-4Einvivowithoutmajoreffectsoncellviability,growth,andmating.Thisfindingprovidesastartingpointfornewexperimentalstrategiestoinvestigatethestructure-functionrelationshipofeukaryotictranslationinitiationfactoreIF-4E.

参考文献：